Artifical Insemination
by the I.C.S.B. Method
Frequently
Asked Questions

Q. How
many units do I need for a successful breeding?
A. Only one!
Each unit holds a minimum of 250 million sperm, more than adequate
for a successful conception.
Q. Can
I breed my bitch by using the unit at any time during the estrus
of my bitch?
A. No! To
ensure success and conception, the bitch's estrus cycle should
be charted using progesterone tests to determine when ovulation
occurs and the bitch inseminated accordingly. This would be decided
by your Veterinarian.
Q. Can
I deposit the unit of semen in the vagina the same way as natural
or chilled semen?
A. No! Chilled
and natural semen collections do not have an extender added to
the collection, as does frozen. The extender used in the frozen
semen cannot overcome the natural mucous at the entrance to the
cervix. Modern scientific methods dictate the protocol to deposit
the unit of semen directly into the uterus. This can be done
by a minor surgical incision, or by the use of a pipette through
the vagina and cervix. This second method is used frequently
in Europe. Either method ensures that the semen is deposited
directly where it is needed, in the uterus. In this way the breeder
has a 98% chance of success.
Q. How
do I know there are actually 250 million sperm in each unit?
A. When the
collection is made, the semen is checked microscopically and
by the computer to see how many good, virile, vigorous, and straight
swimming sperm there are. Based on this information, the sperm
are frozen into pellets and the pellets placed in an ampoule
(one breeding unit) to give 250 million sperm per ampoule. Then
as a live test, a few of the pellets are thawed to see how many
in real-time use would come back to life. Depending on the thaw-back
rate, the ampoules are then adjusted to ensure that upon thaw-back
there would be actually a minimum of 250 million live sperm.
Q. Can
I use more than one unit at the time of breeding?
A. Yes! Definitely!
But bear in mind that based upon the previous answers, the use
of more than one may be unnecessary and excessive, why not wait
and use the others at further breedings. If the ovulation timing
of the bitch is correctly indicated by your veterinarian then
the use of one ampoule is sufficient. Some veterinarians may
want to deposit a second ampoule approximately one day after
the first insemination of the bitch but if the progesterone tests
are done correctly a second deposition is not necessary.
Q. Is
there any chance my bitch can pick up infections or transmissible
diseases from the frozen semen?
A. No! Definitely
Not! The male from whom the collection is made has to have negative
blood test results for Brucellosis and Leptospirosis to ensure
that there are no fears of possible transmission. The blood test
results last for thirty days. After that, any subsequent collections
must be preceded by yet another blood test.
The stud dog is also fully examined by the collecting vet
to ensure it is in optimum health and all vaccinations are in
order.
Q. How
long can I keep this frozen semen?
A. Indefinitely.
The semen can be stored and used in later generations to pick
up certain traits long after the dog itself has died.
Q. Who
owns the semen?
A. Once the
semen is paid for and the shipment has been sent, the accompanying
documentation ensures that the semen is the property of the purchaser
- for ever.
Q. If
I buy some of this semen, can I sell it to someone else?
A. Depending
on the contract with the supplier, the answer is usually "YES".
It should be borne in mind that international agreement
at the moment prohibits it from being transferred via another
country. For example: If a Canadian bought semen from an Englishman,
and then later wished to sell it, he could to another Canadian,
but not to an Australian. The semen can only travel in one straight
line.
This may ultimately change but that is the rule at the
moment.
Q. Can
I have frozen semen from more than one dog shipped in one container
to save cost?
A. Yes! Definitely!
The ampoules are collected, frozen, heat sealed and marked with
the donor's name and credentials. There is no problem in using
the dry shipper to carry the frozen semen of more than one dog.
Q. Is
the semen shipped in liquid nitrogen which causes some airlines
and customs problems? Is this a dangerous cargo?
A. No. To
avoid the problems of some country's attitude towards liquid
nitrogen as a dangerous cargo, the semen is sent in a special
shipper, which is lined with a specific type of foam material
that absorbs and holds the liquid nitrogen so there is no loose
liquid to move around or spill. This is acceptable by the airlines
and ensures prompt and safe delivery.
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